Invitrogen

Vitronectin Monoclonal Antibody (HV2)

Vitronectin Antibody in Immunocytochemistry (ICC/IF)

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Vitronectin Antibody (CSI 003-02-02) in ICC/IF

Immunofluorescence analysis of Vitronectin was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Anti-Vitronectin Mouse Monoclonal Antibody (Product # CSI-003-0202) at 1:200 dilution in 0.1% BSA, incubated ... View More

Vitronectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Vitronectin Antibody in Western Blot (WB)

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产品信息

CSI 003-02-02

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:200

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

HV2

抗原

Human vitronectin purified from plasma by heparin-affinity chromatography.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_1088278

产品详细信息

CSI 003-02-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 003-02-02 is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse).

CSI 003-02-02 has been successfully used in ELISA, immunohistochemistry, and Western blotting techniques. CSI 003-02-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-02-02. It does not interfere with the binding of any known vitronectin ligands.

The epitope of CSI 003-02-02 is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

靶标信息

VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

仅用于科研。不用于诊断过程。未经明确授权不得转售。